Department of Biochemistry

The Laboratory of Biochemistry began its activities in 1969 as part of the Department of Liver and Pancreatic Diseases of the Institute under the leadership of Dr. Rafes YI, who made a significant contribution to the development of theoretical and practical gastroenterology, clinical biochemistry.

From 1974 to 2000 the laboratory was headed by Doctor of Biological Sciences Sheleketina II Under her leadership, for the first time in the country, the effect of digestive hormones on bile secretion was studied, the pathogenesis of biliary secretion disorders in diseases of the biliary tract was studied, with the participation of her followers Cand. Biol. Sciences Averyanova LP and Cand. biol. Domashneva NO, senior researcher Lelyukhina OV created and tested new drugs

2000 – 2007 – Head of the Laboratory Averyanova LP, Candidate of Biological Sciences
2007-2009 – Head of laboratory Domashnevа. N. A. Candidate of Biological Sciences
2010 – 2017 – Head of the Laboratory Klenina Inna Anatoliyivna Candidate of Biological Sciences

 

SCIENTIFIC DIRECTION OF THE DEPARTMENT

  • розробка та вдосконалення біохімічних методів лабораторної діагностики
  • substantiation of a differentiated approach to the development of treatment methods
  • study of pathogenetic mechanisms of diseases of the digestive system

The department performs more than 40 biochemical methods, using modern hardware and software for medical research based on gas chromatograph “Chromatek-Crystal 5000”, biochemical analyzers, spectrophotometric, densitometric, electrophoretic, chromatographic, pH – metric methods

One of the priority areas of biochemical research in the current research topics of the institute is:

  • study of the peculiarities of lipid metabolism (metabolic profile) in biological substrates in order to improve the diagnosis, treatment, as well as provide recommendations for dietary nutrition in diseases of the hepatobiliary system and gastrointestinal tract;
  • determination of the spectrum of short-chain fatty acids in serum and coprofiltrate to assess the state of intestinal microflora, screening and differential diagnosis of intestinal diseases, selection of treatment for patients with diseases of the gastrointestinal tract and its effectiveness, diagnosis of exocrine pancreatic insufficiency (non-invasive diagnostic method)

Opportunities of the unit:

  • gas chromatography of free fatty acids, lipids (short-chain, long-chain) in various biological substrates
  • ascending thin layer chromatography of phospholipids, serum lipids, erythrocyte membranes and bile acids in bile, gastric juice
  • detection of disorders of lipid, protein, carbohydrate metabolism
  • assessment of the activity of the fibrous process
  • detection of intrahepatic cholestasis
  • determination of biochemical parameters of the lipid peroxidation system and antioxidant protection
  • detection of endogenous intoxication
  • assessment of exocrine function of the pancreas
  • assessment of liver detoxification function

 

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